VPS39 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76535
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39. The immunogen is located within amino acids 710 - 760 of VPS39. Amino Acid Squence: HKHYDRNKDGNKDVYLS
Specificity
At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VPS39 Antibody - BSA Free
Western Blot: VPS39 AntibodyBSA Free [NBP1-76535]
Western Blot: VPS39 Antibody [NBP1-76535] - Rat liver tissue lysate with VPS39 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535]
Immunocytochemistry/Immunofluorescence: VPS39 Antibody [NBP1-76535] - Saos2 Cells, anti-VPS39 primary, Hoescht33342, anti-Rabbit alexa594 secondary. Image from verified customer review.Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535] -
Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535] - Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 ug/ml.Green: VPS39 antibody
Red: Phylloidin staining
Blue: DAPI staining
Immunohistochemistry: VPS39 Antibody - BSA Free [NBP1-76535] -
Immunohistochemistry: VPS39 Antibody - BSA Free [NBP1-76535] - Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 u/mL.Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535] -
Immunocytochemistry/ Immunofluorescence: VPS39 Antibody - BSA Free [NBP1-76535] - Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 ug/mL.Applications for VPS39 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
0.5 ug/ml
Reviewed Applications
Read 1 review rated 4 using NBP1-76535 in the following applications:
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VPS39
Alternate Names
hVam6p, KIAA0770FLJ21681, TLP, TRAP1-like protein, vacuolar protein sorting 39 (yeast), vacuolar protein sorting 39 homolog (S. cerevisiae), vam6/Vps39-like protein, VAM6FLJ46546
Gene Symbol
VPS39
UniProt
Additional VPS39 Products
Product Documents for VPS39 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VPS39 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for VPS39 Antibody - BSA Free
Customer Reviews for VPS39 Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
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Application: ImmunofluorescenceSample Tested: Saos2 CellsSpecies: HumanVerified Customer | Posted 03/28/2014Saos2 Cells, anti-VPS39 primary, Hoescht33342, anti-Rabbit alexa594 secondary
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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