VRK1 Antibody

Novus Biologicals | Catalog # NBP1-89548

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VRK1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VRK1 Antibody

VRK1 Antibody Immunocytochemistry/ Immunofluorescence: VRK1 Antibody [VRK1 Antibody]

Immunocytochemistry/ Immunofluorescence: VRK1 Antibody [VRK1 Antibody]

Staining of human cell line A-431 shows localization to nucleus & nucleoli.
Western Blot: 0 [NBP1-89548]

Western Blot: 0 [NBP1-89548]

Western Blot: VRK1 Antibody [NBP1-89548] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-VRK1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548] - Analysis in human testis and skeletal muscle tissues. Corresponding VRK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548]

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.
Immunohistochemistry: VRK1 Antibody [NBP1-89548]

Immunohistochemistry: VRK1 Antibody [NBP1-89548]

Immunohistochemistry: VRK1 Antibody [NBP1-89548] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Applications for VRK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VRK1

VRK1 encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Long Name

Vaccinia-related Kinase 1

Alternate Names

EC 2.7.11.1, MGC117401, MGC138280, MGC142070, PCH1, serine/threonine-protein kinase VRK1, vaccinia related kinase 1, vaccinia virus B1R-related kinase 1, Vaccinia-related kinase 1

Gene Symbol

VRK1

Additional VRK1 Products

Product Documents for VRK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VRK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
p53 Pathway

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Protocols

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