VRL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32096

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VRL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VRL1. The exact sequence is proprietary.

Localization

Cell membrane, Multi-pass membrane protein, Cytoplasm, Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VRL1 Antibody - BSA Free

Western Blot: VRL1 Antibody [NBP1-32096]

Western Blot: VRL1 Antibody [NBP1-32096]

Western Blot: VRL1 Antibody [NBP1-32096] - Unboiled K562 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-32096]

Immunocytochemistry/ Immunofluorescence: VRL1 Antibody [NBP1-32096]

Immunocytochemistry/Immunofluorescence: VRL1 Antibody [NBP1-32096] - 293T cells transfected with vector, pCMV5 3xFlag-mCherry, as positive control. mCherry was co-localized with Flag. 293T cells transfected with vector, pCMV5 3xFlag-TRPV2, as experimental group. TRPV2 was co-localized with Flag at cell membrane. ICC Flag: green TRPV2: red Nucleus: blue mCherry: red fluorescent protein. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-32096]

Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-32096]

Immunohistochemistry-Paraffin: VRL1 Antibody [NBP1-32096] - Analysis of paraffin-embedded human lung, using VRL1 antibody(10 ug/ml).

Applications for VRL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review.

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-32096 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VRL1

This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer. Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Melanosome. Note: Translocates from the cytoplasm to the plasma membrane upon ligand stimulation. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Long Name

Transient receptor potential cation channel subfamily V member 2

Alternate Names

OTRPC2, TrpV2, VRL, VRL-1

Gene Symbol

TRPV2

Additional VRL1 Products

Product Documents for VRL1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VRL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for VRL1 Antibody - BSA Free

Customer Reviews for VRL1 Antibody - BSA Free (1)

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Customer Images

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  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 11/07/2014
    ICC using VRL1 antibody
    VRL1 Antibody - BSA Free NBP1-32096

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