VTA1 Antibody (14G10) - BSA Free
Novus Biologicals | Catalog # NBP2-59420
![Western Blot: VTA1 Antibody (14G10)BSA Free [NBP2-59420]](https://resources.rndsystems.com/images/products/VTA1-Antibody-14G10-Western-Blot-NBP2-59420-img0001.jpg "Western Blot: VTA1 Antibody (14G10)BSA Free [NBP2-59420]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 14G10
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human VTA1 (1-307aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for VTA1 Antibody (14G10) - BSA Free
Western Blot: VTA1 Antibody (14G10)BSA Free [NBP2-59420]
Western Blot: VTA1 Antibody (14G10) [NBP2-59420] - Lane 1: HepG2 cell lysate, Lane 2: 293T cell lysate, Lane 3: HeLa cell lysate, Lane 4: MCF7 cell lysate, Lane 5: A549 cell lysate, Lane 6: Jurkat cell lysate, Lane 7: K562 cell lysate, Lane 8: LnCaP cell lysate, Lane 9: U937 cell lysate, Lane 10: A431 cel![Immunocytochemistry/ Immunofluorescence: VTA1 Antibody (14G10) - BSA Free [NBP2-59420]](https://resources.rndsystems.com/images/products/VTA1-Antibody-14G10-Immunocytochemistry-Immunofluorescence-NBP2-59420-img0002.jpg "Immunocytochemistry/ Immunofluorescence: VTA1 Antibody (14G10) - BSA Free [NBP2-59420]")
Immunocytochemistry/ Immunofluorescence: VTA1 Antibody (14G10) - BSA Free [NBP2-59420]
Immunocytochemistry/Immunofluorescence: VTA1 Antibody (14G10) [NBP2-59420] - Analysis of VTA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VTA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Flow Cytometry: VTA1 Antibody (14G10) - BSA Free [NBP2-59420]](https://resources.rndsystems.com/images/products/VTA1-Antibody-14G10-Flow-Cytometry-NBP2-59420-img0003.jpg "Flow Cytometry: VTA1 Antibody (14G10) - BSA Free [NBP2-59420]")
Flow Cytometry: VTA1 Antibody (14G10) - BSA Free [NBP2-59420]
Flow Cytometry: VTA1 Antibody (14G10) [NBP2-59420] - Flow cytometry analysis of VTA1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for VTA1 Antibody (14G10) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1030
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VTA1
Alternate Names
C6orf55DRG1, Dopamine-responsive gene 1 protein, DRG-1, FLJ27228, homolog of mouse SKD1-binding protein 1, LIP5HSPC228, LYST-interacting protein 5, My012, SBP1chromosome 6 open reading frame 55, SKD1-binding protein 1, vacuolar protein sorting-associated protein VTA1 homolog, Vps20-associated 1 homolog (S. cerevisiae)
Gene Symbol
VTA1
Additional VTA1 Products
Product Documents for VTA1 Antibody (14G10) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VTA1 Antibody (14G10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
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- Preparing Samples for IHC/ICC Experiments
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- Propidium Iodide Cell Viability Flow Cytometry Protocol
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- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
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- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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