WASF2 [p Tyr150] Antibody

Name: WASF2 [p Tyr150] Antibody
Brand: Novus Biologicals
SKU: NBP3-23201
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23201

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all WASF2 Primary Antibodies »

Product Specifications

Immunogen

Phospho-WASF2 (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WASF2. This sequence has high homology with similar regions in rat and mouse WASF2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151). [UniProt# Q9Y6W5]

Modification

p Tyr150

Specificity

This antibody was cross-adsorbed to phospho-WASF2 (Tyr-125) and unphosphorylated WASF2 (Tyr-150) peptides before affinity purification using phospho-WASF2 (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa corresponding to phosphorylated WASF2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for WASF2 [p Tyr150] Antibody

Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] -

Western Blot: WASF2 [p Tyr150] Antibody [NBP3-23201] - Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WASF2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).

Applications for WASF2 [p Tyr150] Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: WASF2

WASF2 encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X.

Alternate Names

IMD2, Protein WAVE-2, SCAR2suppressor of cyclic-AMP receptor (WASP-family), Verprolin homology domain-containing protein 2, WAS protein family, member 2, WASP family Verprolin-homologous protein 2, WAVE2dJ393P12.2, wiskWASP family protein member 2

Gene Symbol

WASF2

Additional WASF2 Products

Product Documents for WASF2 [p Tyr150] Antibody

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Product Specific Notices for WASF2 [p Tyr150] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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