WBP11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38602

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WBP11 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KPQITNPKAEITRFVPTALRVRRENK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WBP11 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WBP11 Antibody [NBP2-38602]

Immunocytochemistry/ Immunofluorescence: WBP11 Antibody [NBP2-38602]

Immunocytochemistry/Immunofluorescence: WBP11 Antibody [NBP2-38602] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry: WBP11 Antibody [NBP2-38602]

Immunohistochemistry: WBP11 Antibody [NBP2-38602]

Immunohistochemistry: WBP11 Antibody [NBP2-38602] - Staining of human heart muscle shows moderate nuclear positivity in myocytes.
Simple Western: WBP11 Antibody [NBP2-38602]

Simple Western: WBP11 Antibody [NBP2-38602]

Simple Western: WBP11 Antibody [NBP2-38602] - Simple Western lane view shows a specific band for WBP11 in 0.2 mg/ml of Ntera-2 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: WBP11 Antibody [NBP2-38602]

Simple Western: WBP11 Antibody [NBP2-38602]

Simple Western: WBP11 Antibody [NBP2-38602] - Electropherogram image of the corresponding Simple Western lane view. WBP11 antibody was used at 1:25 dilution on Ntera-2 lysate(s) respectively.

Applications for WBP11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Ntera-2 lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 134 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WBP11

WBP11 encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq]

Alternate Names

DKFZp779M1063, Npw38-binding protein, Npw38-binding protein NpwBP, NpwBP, NPWBPsplicing factor, PQBP1 and PP1 interacting, SH3 domain-binding protein SNP70, SIPP1WW domain-binding protein 11, SNP70, Splicing factor that interacts with PQBP-1 and PP1, WBP-11, WW domain binding protein 11

Gene Symbol

WBP11

UniProt

Q9Y2W2 (Human)

Additional WBP11 Products

Product Documents for WBP11 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WBP11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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