West Nile Virus Envelope Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56744

Novus Biologicals

Key Product Details

Species Reactivity

Virus

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A synthetic peptide corresponding to 73-86 (CPTMGEAHNEKRAD) of West Nile Virus Envelope glycoprotein (NC_001563).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for West Nile Virus Envelope Antibody - BSA Free

Western Blot: West Nile Virus Envelope Antibody [NB100-56744]

Western Blot: West Nile Virus Envelope Antibody [NB100-56744]

Western Blot: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Detection of West Nile Virus Envelope in (A) untransfected and (B) transfected cells using this antibody.
Immunohistochemistry-Paraffin: West Nile Virus Envelope Antibody [NB100-56744]

Immunohistochemistry-Paraffin: West Nile Virus Envelope Antibody [NB100-56744]

Immunohistochemistry-Paraffin: West Nile Virus Envelope Antibody [NB100-56744] - Analysis using the Azide Free version of NB100-56744. Staining of West Nile Virus Envelope in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.

Applications for West Nile Virus Envelope Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24920798)

Immunohistochemistry

1 ug/ml

Immunohistochemistry-Paraffin

1 ug/ml

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: West Nile Virus Envelope

West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.

UniProt

Additional West Nile Virus Envelope Products

Product Documents for West Nile Virus Envelope Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for West Nile Virus Envelope Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for West Nile Virus Envelope Antibody - BSA Free

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Protocols

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