WHSC1 Antibody (CL1063) - BSA Free

Novus Biologicals | Catalog # NBP2-52963

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL1063

Format

BSA Free
View all WHSC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O96028, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for WHSC1 Antibody (CL1063) - BSA Free

Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963]

Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963]

Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunocytochemistry/ Immunofluorescence: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunocytochemistry/Immunofluorescence: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of U-251 cells using the Anti-WHSC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in a subset of tumor cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human cerebral cortex shows moderate positivity in neuronal cell nuclei.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human testis shows nuclear immunoreactivity in a subset of cells in seminiferous tubules.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human tonsil shows nuclear positivity in the reaction centrum cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963]

Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human ventricular cancer shows strong nuclear immunoreactivity in tumor cells.

Applications for WHSC1 Antibody (CL1063) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WHSC1

WHSC1 encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq]

Alternate Names

EC 2.1.1.43, FLJ23286, IL5 promoter REII region-binding protein, KIAA1090, MGC176638, MMSETNSD2Multiple myeloma SET domain-containing protein, multiple myeloma SET domain containing protein type III, nuclear receptor binding SET domain protein 2, Nuclear SET domain-containing protein 2, probable histone-lysine N-methyltransferase NSD2, Protein trithorax-5, REIIBP, trithorax/ash1-related protein 5, TRX5, WHS, Wolf-Hirschhorn syndrome candidate 1, Wolf-Hirschhorn syndrome candidate 1 protein

Gene Symbol

NSD2

Additional WHSC1 Products

Product Documents for WHSC1 Antibody (CL1063) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for WHSC1 Antibody (CL1063) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for WHSC1 Antibody (CL1063) - BSA Free

There are currently no reviews for this product. Be the first to review WHSC1 Antibody (CL1063) - BSA Free and earn rewards!

Have you used WHSC1 Antibody (CL1063) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies