WHSC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82633

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WHSC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

152 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WHSC1 Antibody - BSA Free

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633]

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633]

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining in human testis and liver tissues using anti-NSD2 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: WHSC1 Antibody [NBP1-82633]

Immunocytochemistry/ Immunofluorescence: WHSC1 Antibody [NBP1-82633]

Immunocytochemistry/Immunofluorescence: WHSC1 Antibody [NBP1-82633] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633]

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633]

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633]

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633]

Immunohistochemistry-Paraffin: WHSC1 Antibody [NBP1-82633] - Staining of human testis shows high expression.

Applications for WHSC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICCIF Fixation Permeabilization: Use PFA/Triton X-100. WB, ICC/IF reported in the scientific literature (PMID: 26771714).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WHSC1

WHSC1 encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq]

Alternate Names

EC 2.1.1.43, FLJ23286, IL5 promoter REII region-binding protein, KIAA1090, MGC176638, MMSETNSD2Multiple myeloma SET domain-containing protein, multiple myeloma SET domain containing protein type III, nuclear receptor binding SET domain protein 2, Nuclear SET domain-containing protein 2, probable histone-lysine N-methyltransferase NSD2, Protein trithorax-5, REIIBP, trithorax/ash1-related protein 5, TRX5, WHS, Wolf-Hirschhorn syndrome candidate 1, Wolf-Hirschhorn syndrome candidate 1 protein

Entrez Gene IDs

7468 (Human)

Gene Symbol

NSD2

UniProt

O96028 (Human)

Additional WHSC1 Products

Product Documents for WHSC1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WHSC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WHSC1 Antibody - BSA Free

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Protocols

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