WHSC2 Antibody (6B11H8) - BSA Free

Novus Biologicals | Catalog # NBP2-37394

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 6B11H8

Format

BSA Free
View all WHSC2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WHSC2 Antibody (6B11H8) - BSA Free

Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate.
Immunocytochemistry/ Immunofluorescence: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunocytochemistry/ Immunofluorescence: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunocytochemistry/Immunofluorescence: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunohistochemistry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.
Flow Cytometry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Flow Cytometry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Flow Cytometry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Flow cytometric analysis of HEK293 cells using WHSC2 mouse mAb (green) and negative control (purple).
Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mAb against human WHSC2 recombinant protein. (Expected MW is 50.2 kDa)
Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8)BSA Free [NBP2-37394]

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mAb against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunohistochemistry: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WHSC2 mouse mAb with DAB staining.
ELISA: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

ELISA: WHSC2 Antibody (6B11H8) - BSA Free [NBP2-37394]

ELISA: WHSC2 Antibody (6B11H8) [NBP2-37394] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for WHSC2 Antibody (6B11H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WHSC2

WHSC2 is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq]

Alternate Names

FLJ10442, negative elongation factor A, NELFA, NELF-AFLJ25112, Wolf-Hirschhorn syndrome candidate 2, Wolf-Hirschhorn syndrome candidate 2 protein

Gene Symbol

NELFA

Additional WHSC2 Products

Product Documents for WHSC2 Antibody (6B11H8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for WHSC2 Antibody (6B11H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for WHSC2 Antibody (6B11H8) - BSA Free

There are currently no reviews for this product. Be the first to review WHSC2 Antibody (6B11H8) - BSA Free and earn rewards!

Have you used WHSC2 Antibody (6B11H8) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies