Withaferin A (CAS 5119-48-2): R&D Systems
Catalog Number: 2816
Chemical Name: (4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
Biological Activity
Steroid lactone that displays anti-inflammatory, antitumor and antiangiogenic activity. Inhibits endothelial cells (HUVEC) sprouting in vitro (IC50 = 12 nM) and in vivo. Prevents NF-κB activation by inhibiting activation of IKKβ. Also inhibits chymotrypsin-like activity of the 20S proteasome.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 10 mM in DMSO and to 5 mM in ethanol
  • Purity:
  • Storage:
    Desiccate at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Withaferin A is a potent inhibitor of angiogenesis.
    Mohan et al.
    Angiogenesis, 2004;7:115
  2. The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from "Indian Winter Cherry".
    Yang et al.
    Mol.Pharmacol., 2007;71:426
  3. Withaferin A strongly elicits IkB kinase β hyperphosphorylation concomitant with potent inhibition of its kinase activity.
    Kaileh et al.
    J.Biol.Chem., 2007;282:4253

The citations listed below are publications that use Tocris products. Selected citations for Withaferin A include:

4 Citations: Showing 1 - 4
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  1. The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages.
    Authors: Yang Et al.
    Neural Plast 2016;7:11385
  2. Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes.
    Authors: Martorana Et al.
    EMBO J 2015;2015:381964
  3. TAK-1/p38/nNFκB signaling inhibits myoblast differentiation by increasing levels of Activin A.
    Authors: Trendelenburg Et al.
    Skelet Muscle 2012;2:3
  4. Celastrol suppresses invasion of colon and pancreatic cancer cells through the downregulation of expression of CXCR4 chemokine receptor.
    Authors: Yadav Et al.
    J Mol Med (Berl) 2010;88:1243

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