WNK1 Antibody (HL2184) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25748
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2184 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragmemt of human WNK1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for WNK1 Antibody (HL2184) - Azide and BSA Free
Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Immunohistochemistry-Paraffin: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] -
Immunohistochemistry-Paraffin: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for WNK1 Antibody (HL2184) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WNK1
WNK1 mutations have been associated with Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II) and congenital sensory neuropathy.
WNK1 is also known as Human Accelerated Region 5, and is thought to have played a key role in differentiating humans from apes.
Long Name
WNK Lysine-deficient Protein Kinase 1
Alternate Names
KDP, PHA2C, PRKWNK1
Gene Symbol
WNK1
Additional WNK1 Products
Product Documents for WNK1 Antibody (HL2184) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WNK1 Antibody (HL2184) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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