Wnt-2 Antibody (HL1999) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25752

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1999 expressed in HEK293

Format

Azide and BSA Free
View all Wnt-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Wnt-2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-2 Antibody (HL1999) - Azide and BSA Free

Wnt-2 Antibody (HL1999) - Azide and BSA Free

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] -

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-2 Antibody (HL1999) - Azide and BSA Free

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] -

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-2 Antibody (HL1999) - Azide and BSA Free

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] -

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-2 Antibody (HL1999) - Azide and BSA Free

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] -

Western Blot: Wnt-2 Antibody (HL1999) - Azide and BSA Free [NBP3-25752] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt2 antibody [HL1999] (NBP3-25752) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Wnt-2 Antibody (HL1999) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Wnt-2 Antibody (HL1999) [NBP3-25752] -

Wnt-2 antibody [HL1999] detects Wnt-2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt-2 stained by Wnt-2 antibody [HL1999] (NBP3-25752) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Wnt-2 Antibody (HL1999) - Azide and BSA Free

Western Blot: Wnt-2 Antibody (HL1999) [NBP3-25752] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Wnt-2 antibody [HL1999] (NBP3-25752) diluted at 1:7500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Wnt-2 Antibody (HL1999) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-2

The WNT2 gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulat

Long Name

Wingless-type MMTV Integration Site Family Member 2

Alternate Names

INT1L1, IRP, Wnt2

Gene Symbol

WNT2

Additional Wnt-2 Products

Product Documents for Wnt-2 Antibody (HL1999) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Wnt-2 Antibody (HL1999) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

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