Wnt-5a Antibody (CL13683) - BSA Free

Novus Biologicals | Catalog # NBP3-24567

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL13683

Format

BSA Free
View all Wnt-5a Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P41221, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Wnt-5a Antibody (CL13683) - BSA Free

Immunohistochemistry-Paraffin: Wnt-5a Antibody (CL13683) [NBP3-24567]

Staining of human salivary gland shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Wnt-5a Antibody (CL13683) [NBP3-24567]

Staining of human cerebellum shows no positivity in Purkinje cells as expected.

Immunohistochemistry-Paraffin: Wnt-5a Antibody (CL13683) [NBP3-24567]

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Wnt-5a Antibody (CL13683) [NBP3-24567]

Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Applications for Wnt-5a Antibody (CL13683) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody (CL13683) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Wnt-5a Antibody (CL13683) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Ligands (Wnts)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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