WT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31314

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Zebrafish

Cited:

Mouse, Rat

Predicted:

Chicken (91%), Human (100%), Porcine (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human WT1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Japanese medaka (81%), Xenopus laevis (85%). Rat reactivity reported in scientific literature (PMID: 7926762).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WT1 Antibody - BSA Free

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse liver. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
WT1 Antibody

Western Blot: WT1 Antibody [NBP1-31314] -

Western Blot: WT1 Antibody [NBP1-31314] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with wt1a + wt1b antibody (NBP1-31314) diluted at 1:1000.

Applications for WT1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WT1

Wilm's Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilm's tumors and highly expressed in a wide variety of other malignancies (1). It is believed that WT in its two isoforms acts as both a transcription factor and a post-transcriptional regulator that binds RNA and can interact with splicing components (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome (3).

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

Additional WT1 Products

Product Documents for WT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WT1 Antibody - BSA Free

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Protocols

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FAQs

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