WW domain binding protein 5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84208

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WW domain binding protein 5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGKPENESEPKHEEEPKPEEKPEEEEKLEEEAKAKGTFRERLIQSLQEFKEDIHNRHLSNEDMFREVDEIDEIRRVRNKLIVMRWKVNRN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WW domain binding protein 5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: WW domain binding protein 5 Antibody [NBP1-84208]

Immunocytochemistry/ Immunofluorescence: WW domain binding protein 5 Antibody [NBP1-84208]

Immunocytochemistry/Immunofluorescence: WW domain binding protein 5 Antibody [NBP1-84208] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
WW domain binding protein 5 Antibody - BSA Free Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Staining of human fallopian tube shows strong positivity in cilia in glandular cells.
WW domain binding protein 5 Antibody - BSA Free Western Blot: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Western Blot: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Analysis in control (vector only transfected HEK293T lysate) and WBP5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414074).
WW domain binding protein 5 Antibody - BSA Free Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Staining of human lymph node shows no positivity in non-germinal center cells as expected.
WW domain binding protein 5 Antibody - BSA Free Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
WW domain binding protein 5 Antibody - BSA Free Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody - BSA Free [NBP1-84208]

Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Applications for WW domain binding protein 5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WW domain binding protein 5

The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein. This gene also encodes a domain with similarity to the transcription elongation factor A, SII-related family. Alternative splicing results in multiple transcript variants encoding a single isoform. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 through 4 encode the same isoform.

Alternate Names

DKFZp313K1940, N4, NDFIP1, pp21 homolog, TCEAL9, WBP-5, WW domain binding protein 1, WW domain binding protein 5, WW domain-binding protein 5

Gene Symbol

TCEAL9

Additional WW domain binding protein 5 Products

Product Documents for WW domain binding protein 5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WW domain binding protein 5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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