XPC Antibody (3.26) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-477

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3.26

Format

Azide and BSA Free
View all XPC Primary Antibodies »

Product Specifications

Immunogen

Recombinant XPC purified from E. coli

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17229834). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XPC Antibody (3.26) - Azide and BSA Free

Western Blot: XPC Antibody (3.26) [NB100-477]

Western Blot: XPC Antibody (3.26) [NB100-477]

XPC-Antibody-3-26-Western-Blot-NB100-477-img0007.jpg
Western Blot: XPC Antibody (3.26) [NB100-477]

Western Blot: XPC Antibody (3.26) [NB100-477]

Western Blot: XPC Antibody (3.26) [NB100-477] - HeLa whole cell lysate detected using rabbit polyclonal.
Western Blot: XPC Antibody (3.26) [NB100-477]

Western Blot: XPC Antibody (3.26) [NB100-477]

Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.
XPC Antibody (3.26) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: XPC Antibody (3.26) [NB100-477] -

XPC antibody [3.26] detects XPC protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPC protein stained by XPC antibody [3.26] (NB100-477) diluted at 1:100.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 ?m.

Applications for XPC Antibody (3.26) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-P (Paraffin sections) Assay dependent

Reviewed Applications

Read 1 review rated 5 using NB100-477 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XPC

Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair.

Alternate Names

complementation group C antibody, p125, RAD4, Xeroderma pigmentosum group C-complementing protein, xeroderma pigmentosum, complementation group C, XP3, XPCCDNA repair protein complementing XP-C cells

Entrez Gene IDs

7508 (Human)

Gene Symbol

XPC

UniProt

Q01831 (Human)

Additional XPC Products

Product Documents for XPC Antibody (3.26) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for XPC Antibody (3.26) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for XPC Antibody (3.26) - Azide and BSA Free

Customer Reviews for XPC Antibody (3.26) - Azide and BSA Free (1)

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Customer Images

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  • XPC Antibody (3.26)
    Name: Darshil Patel
    Application: Western Blot
    Sample Tested: MEFs whole cell lysate
    Species: Mouse
    Verified Customer | Posted 07/18/2017
    XPC Antibody (3.26) - Azide and BSA Free NB100-477

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Protocols

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