XRN2 Antibody

Novus Biologicals | Catalog # NB100-57541

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all XRN2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 900 and 950 human 5'-3' exoribonuclease 2 using the numbering given in entry NP_036387.2 (GeneID 22803).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRN2 Antibody

Western Blot: XRN2 Antibody [NB100-57541]

Western Blot: XRN2 Antibody [NB100-57541]

Western Blot: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541]

Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541]

Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB
Western Blot: XRN2 Antibody [NB100-57541]

Western Blot: XRN2 Antibody [NB100-57541]

Western Blot: XRN2 Antibody [NB100-57541] - Detection of mouse XRN2 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541]

Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541]

Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB
Immunoprecipitation: XRN2 Antibody [NB100-57541]

Immunoprecipitation: XRN2 Antibody [NB100-57541]

Immunoprecipitation: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for IP at 3 ug per reaction. XRN2 was also immunoprecipitated by another rabbit anti-XRN2 antibody. For blotting immunoprecipitated XRN2, NB100-57541 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for XRN2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:10000 - 1:25000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB100-57541 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XRN2

XRN2 is a 5'-to-3' exoribonuclease involved in 3'-end mRNA processing. XRN2 is important for transcription termination and is responsible for degrading the downstream 3'-end-cleaved RNA. Recent studies show that XRN2 is recruited to 3'-end processing machinery by p54nrb/PSF. Alternate names for XRN2 include 5'-3' exoribonuclease 2, DHM1-like protein, and DHP protein.

Alternate Names

5'-3' exoribonuclease 2, DHM1-like protein, Dhm1-like protein (mouse homolog), DHP protein, EC 3.1.13, EC 3.1.13.-

Entrez Gene IDs

22803 (Human)

Gene Symbol

XRN2

UniProt

Q9H0D6 (Human)

Additional XRN2 Products

Product Documents for XRN2 Antibody

Certificate of Analysis

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Product Specific Notices for XRN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for XRN2 Antibody (1)

4 out of 5
1 Customer Rating
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  • Name: Stephanie Moon
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 01/07/2014

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