XRN2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13529

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: NSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGVQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRN2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: XRN2 Antibody [NBP2-13529]

Immunocytochemistry/ Immunofluorescence: XRN2 Antibody [NBP2-13529]

Immunocytochemistry/Immunofluorescence: XRN2 Antibody [NBP2-13529] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: XRN2 Antibody [NBP2-13529]

Immunohistochemistry-Paraffin: XRN2 Antibody [NBP2-13529]

Immunohistochemistry-Paraffin: XRN2 Antibody [NBP2-13529] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
XRN2 Antibody - BSA Free Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Staining of human Skin shows strong nuclear positivity in squamous epithelial cells.
XRN2 Antibody - BSA Free Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.
XRN2 Antibody - BSA Free Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Staining of human Colon shows strong nuclear positivity in glandular cells.
XRN2 Antibody - BSA Free Western Blot: XRN2 Antibody - BSA Free [NBP2-13529]

Western Blot: XRN2 Antibody - BSA Free [NBP2-13529]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
XRN2 Antibody - BSA Free Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Immunohistochemistry: XRN2 Antibody - BSA Free [NBP2-13529]

Staining of human Lymph node shows strong nuclear positivity in germinal and non-germinal center cells.

Applications for XRN2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunofluorescence, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XRN2

XRN2 is a 5'-to-3' exoribonuclease involved in 3'-end mRNA processing. XRN2 is important for transcription termination and is responsible for degrading the downstream 3'-end-cleaved RNA. Recent studies show that XRN2 is recruited to 3'-end processing machinery by p54nrb/PSF.

Alternate Names

5'-3' exoribonuclease 2, DHM1-like protein, Dhm1-like protein (mouse homolog), DHP protein, EC 3.1.13, EC 3.1.13.-

Gene Symbol

XRN2

Additional XRN2 Products

Product Documents for XRN2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for XRN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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