YAP1 Antibody (SU33-06)

Novus Biologicals | Catalog # NBP2-67467

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SU33-06 expressed in HEK293
View all YAP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human YAP1 aa 421-470 / 504. (SwissProt: P46937 Human)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YAP1 Antibody (SU33-06)

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution 1:50-1:200.
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunocytochemistry/Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467] - Staining YAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Dilution 1:50-1:200.
Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of YAP1 on HepG2 cell lysates using anti-YAP1 antibody at 1/1,000 dilution. Observed molecular weight 65 kDa.
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunocytochemistry/Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467] - ICC staining YAP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Dilution 1:50-1:200.
Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution 1:50-1:200.
Flow Cytometry: YAP1 Antibody (SU33-06) [NBP2-67467]

Flow Cytometry: YAP1 Antibody (SU33-06) [NBP2-67467]

Flow Cytometry: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of NIH/3T3 cells with YAP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467]

Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution1:50-1:200.
YAP1 Antibody (SU33-06) Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western blot analysis on different lysates with NBP2-67467 at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane) Lane 2: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67467 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
YAP1 Antibody (SU33-06) Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western blot analysis on different lysates with NBP2-67467 at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67467, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54kDa Observed band size: 70kDa
YAP1 Antibody (SU33-06) Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467]

Western blot analysis on different lysates with NBP2-67467 at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse testis tissue lysate Lane 4: Mouse skin tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 54 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67467 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for YAP1 Antibody (SU33-06)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YAP1

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Gene Symbol

YAP1

Additional YAP1 Products

Product Documents for YAP1 Antibody (SU33-06)

Certificate of Analysis

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Product Specific Notices for YAP1 Antibody (SU33-06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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