YY1 Antibody (2C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00007528-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C4

Format

Azide and BSA Free
View all YY1 Primary Antibodies »

Product Specifications

Immunogen

YY1 (NP_003394, 221 a.a. ~ 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTH

Specificity

YY1 - YY1 transcription factor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for YY1 Antibody (2C4) - Azide and BSA Free

Western Blot: YY1 Antibody (2C4) [H00007528-M01]

Western Blot: YY1 Antibody (2C4) [H00007528-M01]

Western Blot: YY1 Antibody (2C4) [H00007528-M01] - YY1 monoclonal antibody (M01), clone 2C4 Analysis of YY1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: YY1 Antibody (2C4) [H00007528-M01]

Immunocytochemistry/ Immunofluorescence: YY1 Antibody (2C4) [H00007528-M01]

Immunocytochemistry/Immunofluorescence: YY1 Antibody (2C4) [H00007528-M01] - Analysis of monoclonal antibody to YY1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: YY1 Antibody (2C4) [H00007528-M01]

Immunohistochemistry-Paraffin: YY1 Antibody (2C4) [H00007528-M01]

Immunohistochemistry-Paraffin: YY1 Antibody (2C4) [H00007528-M01] - Analysis of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Western Blot: YY1 Antibody (2C4) [H00007528-M01]

Western Blot: YY1 Antibody (2C4) [H00007528-M01]

Western Blot: YY1 Antibody (2C4) [H00007528-M01] - Analysis of YY1 expression in transfected 293T cell line by YY1 monoclonal antibody (M01), clone 2C4.Lane 1: YY1 transfected lysate(44.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: YY1 Antibody (2C4) [H00007528-M01] - Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.

Applications for YY1 Antibody (2C4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: YY1

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Entrez Gene IDs

7528 (Human)

Gene Symbol

YY1

OMIM

600013 (Human)

UniProt

NP_003394 (Human)

Additional YY1 Products

Product Documents for YY1 Antibody (2C4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for YY1 Antibody (2C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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