ZBTB20 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20936

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZBTB20 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZBTB20. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZBTB20 Antibody - BSA Free

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936]

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936]

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936] - Mouse hippocampus. ZBTB20 stained by ZBTB20 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunocytochemistry/ Immunofluorescence: ZBTB20 Antibody [NBP2-20936]

Immunocytochemistry/ Immunofluorescence: ZBTB20 Antibody [NBP2-20936]

Immunocytochemistry/Immunofluorescence: ZBTB20 Antibody [NBP2-20936] - ZBTB20 antibody detects ZBTB20 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZBTB20 stained by ZBTB20 antibody diluted at 1:500. Red: Tau, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936]

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936]

Immunohistochemistry-Paraffin: ZBTB20 Antibody [NBP2-20936] - Human breast cancer, using ZBTB20 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
ZBTB20 Antibody - BSA Free

Western Blot: ZBTB20 Antibody [NBP2-20936] -

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
NBP2-20936 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ZBTB20 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZBTB20 Antibody [NBP2-20936] -

ZBTB20 antibody detects ZBTB20 protein at by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZBTB20 stained by ZBTB20 antibody (NBP2-20936) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
ZBTB20 Antibody - BSA Free

Western Blot: ZBTB20 Antibody [NBP2-20936] -

HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZBTB20 antibody (NBP2-20936) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ZBTB20 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Knockdown Validated

Reported in scientific publication (PMID: 32363359)

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZBTB20

ZBTB20 - zinc finger and BTB domain containing 20

Alternate Names

BTB/POZ zinc finger protein DPZF, dendritic cell-derived BTB/POZ zinc finger, Dendritic-derived BTB/POZ zinc finger protein, DPZFZinc finger protein 288ZNF288DKFZp566F123, FLJ26458, HOF, ODA-8S, zinc finger and BTB domain containing 20, zinc finger and BTB domain-containing protein 20

Gene Symbol

ZBTB20

Additional ZBTB20 Products

Product Documents for ZBTB20 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZBTB20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for ZBTB20 Antibody - BSA Free

Customer Reviews for ZBTB20 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ZBTB20 Antibody - BSA Free and earn rewards!

Have you used ZBTB20 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies