ZEB1 Antibody (4C4) - Azide and BSA Free
Novus Biologicals | Catalog # H00006935-M01
![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00006935-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4C4
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
ZEB1 (NP_110378, 801 a.a. ~ 900 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTE
Marker
Mesenchymal Cells Marker
Specificity
transcription factor 8 (represses interleukin 2 expression)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ZEB1 Antibody (4C4) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00006935-M01-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on HeLa cell. Antibody concentration 10 ug/ml.![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00006935-M01-img0003.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on U-2 OS cell. Antibody concentration 10 ug/ml.![ELISA: ZEB1 Antibody (4C4) [H00006935-M01]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-4C4-ELISA-H00006935-M01-img0006.jpg "ELISA: ZEB1 Antibody (4C4) [H00006935-M01]")
ELISA: ZEB1 Antibody (4C4) [H00006935-M01]
ELISA: ZEB1 Antibody (4C4) [H00006935-M01] - Detection limit for recombinant GST tagged ZEB1 is 3 ng/ml as a capture antibody.Applications for ZEB1 Antibody (4C4) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Entrez Gene IDs
6935 (Human)
Gene Symbol
ZEB1
OMIM
189909 (Human)
UniProt
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (4C4) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZEB1 Antibody (4C4) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ZEB1 Antibody (4C4) - Azide and BSA Free
Powered by Bioz
Customer Reviews for ZEB1 Antibody (4C4) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review ZEB1 Antibody (4C4) - Azide and BSA Free and earn rewards!
Have you used ZEB1 Antibody (4C4) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ZEB1 Antibody (4C4) - Azide and BSA Free
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
Loading...