ZEB1 Antibody (CL0151) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43907
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0151
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P37275, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ZEB1 Antibody (CL0151) - Azide and BSA Free
![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512135871.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]
Staining in A549 cell line with Anti-ZEB1 monoclonal antibody) staining in green. Microtubule probes are visualized in red (where available).![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512173122.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]")
![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512163612.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP3-43907]
Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody) staining in green. Microtubule probes are visualized in red (where available).![ZEB1 Antibody (CL0151) - Azide and BSA Free Western Blot: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512910.jpg "Western Blot: ZEB1 Antibody [NBP3-43907]")
Western Blot: ZEB1 Antibody [NBP3-43907]
Lane 1: Marker [kDa]Lane 2: Human cell line RH-30
Lane 3: Human cell line CACO-2
![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512303816.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]")
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]
Staining of human endometrium shows strong nuclear positivity in stromal cells.![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512303840.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]")
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]
Staining of human cerebral cortex shows strong nuclear positivity in neurons.![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202512362413.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]")
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]
Staining of human liver shows no nuclear positivity in hepatocytes as expected.![ZEB1 Antibody (CL0151) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-282202513314613.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]")
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP3-43907]
Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.![ZEB1 Antibody (CL0151) - Azide and BSA Free Western Blot: ZEB1 Antibody [NBP3-43907]](https://resources.rndsystems.com/images/products/nbp3-43907_mouse-zeb1-mab-cl0151-azide-and-bsa-free-28220251337826.jpg "Western Blot: ZEB1 Antibody [NBP3-43907]")
Applications for ZEB1 Antibody (CL0151) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Gene Symbol
ZEB1
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (CL0151) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZEB1 Antibody (CL0151) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ZEB1 Antibody (CL0151) - Azide and BSA Free
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Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
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