ZEB1 Antibody (CL0151) - BSA Free
Novus Biologicals | Catalog # NBP2-52866
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0151
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P37275, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ZEB1 Antibody (CL0151) - BSA Free
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg "Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]")
Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]
Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg "Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]")
Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]
Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody stianing shown in green.![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]
Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]
Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.rndsystems.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]
Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.Applications for ZEB1 Antibody (CL0151) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Gene Symbol
ZEB1
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (CL0151) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZEB1 Antibody (CL0151) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ZEB1 Antibody (CL0151) - BSA Free
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Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
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