ZER1 Antibody (HL3491)

Novus Biologicals | Catalog # NBP3-48820

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3491
View all ZER1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human ZER1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZER1 Antibody (HL3491)

ZER1 Antibody (HL3491)

Western Blot: ZER1 Antibody (HL3491) [NBP3-48820] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZER1 antibody [HL3491] (NBP3-48820) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ZER1 Antibody (HL3491)

Immunocytochemistry/ Immunofluorescence: ZER1 Antibody (HL3491) [NBP3-48820] -

ZER1 antibody [HL3491] detects ZER1 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZER1 stained by ZER1 antibody [HL3491] (NBP3-48820) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
ZER1 Antibody (HL3491)

Western Blot: ZER1 Antibody (HL3491) [NBP3-48820] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZER1 antibody [HL3491] (NBP3-48820) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for ZER1 Antibody (HL3491)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZER1

Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZER1-CUL2-Elongin BC

Alternate Names

C9orf60, chromosome 9 open reading frame 60, homolog of Zyg-11, Hzygzyg-11 homolog B (C. elegans)-like, protein zer-1 homolog, zer-1 homolog (C. elegans), ZYG homolog, Zyg-11 homolog B-like protein, ZYG11BL, ZYGRP11-545E17.4

Gene Symbol

ZER1

Additional ZER1 Products

Product Documents for ZER1 Antibody (HL3491)

Certificate of Analysis

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Product Specific Notices for ZER1 Antibody (HL3491)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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