ZNF462 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-78192
![Western Blot: ZNF462 Antibody [NBP1-78192]](https://resources.rndsystems.com/images/products/ZNF462-Antibody-Western-Blot-NBP1-78192-img0009.jpg "Western Blot: ZNF462 Antibody [NBP1-78192]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 2456 and 2506 of human Zinc Finger Protein 462 using the numbering given in entry NP_067047.4 (GeneID 58499).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF462 Antibody - BSA Free
Western Blot: ZNF462 Antibody [NBP1-78192]
Western Blot: ZNF462 Antibody [NBP1-78192] - Detection of Human ZNF462 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody NBP1-78192 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: ZNF462 Antibody [NBP1-78192]](https://resources.rndsystems.com/images/products/ZNF462-Antibody-Immunohistochemistry-Paraffin-NBP1-78192-img0007.jpg "Immunohistochemistry-Paraffin: ZNF462 Antibody [NBP1-78192]")
Immunohistochemistry-Paraffin: ZNF462 Antibody [NBP1-78192]
Immunohistochemistry-Paraffin: ZNF462 Antibody [NBP1-78192] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- ZNF462 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.![Western Blot: ZNF462 Antibody [NBP1-78192]](https://resources.rndsystems.com/images/products/ZNF462-Antibody-Western-Blot-NBP1-78192-img0006.jpg "Western Blot: ZNF462 Antibody [NBP1-78192]")
Western Blot: ZNF462 Antibody [NBP1-78192]
Western Blot: ZNF462 Antibody [NBP1-78192] - NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: ZNF462 Antibody [NBP1-78192]](https://resources.rndsystems.com/images/products/ZNF462-Antibody-Immunoprecipitation-NBP1-78192-img0010.jpg "Immunoprecipitation: ZNF462 Antibody [NBP1-78192]")
Immunoprecipitation: ZNF462 Antibody [NBP1-78192]
Immunoprecipitation: ZNF462 Antibody [NBP1-78192] - Detection of human ZNF462 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody NBP1-78192 (lot NBP1-78192-2) used for IP at 6 ug per reaction. ZNF462 was also immunoprecipitated by a previous lot of this antibody NBP1-78192-1. For blotting immunoprecipitated ZNF462, NBP1-78192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for ZNF462 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 -1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2-10 mcg/mg
Western Blot
1:1000-1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZNF462
Alternate Names
BAZ 1, BAZ1, BAZ-1, BWSCR2-associated zinc finger protein 1, zinc finger protein 214
Gene Symbol
ZNF462
UniProt
Additional ZNF462 Products
Product Documents for ZNF462 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZNF462 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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