The cell-surface transmembrane E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) and its homologue ring finger 43 (RNF43) are negative feedback regulators of Wnt signaling. ZNRF3 is associated with the Wnt receptor complex, and inhibits Wnt signaling by promoting the turnover of frizzled and LRP6. R-spondin may enhance Wnt signaling by inhibiting ZNRF3. ZNRF3 may be a target for therapeutic exploration.
ZNRF3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-03924
![Western Blot: ZNRF3 AntibodyAzide and BSA Free [NBP3-03924]](https://resources.rndsystems.com/images/products/ZNRF3-Antibody-Western-Blot-NBP3-03924-img0006.jpg "Western Blot: ZNRF3 AntibodyAzide and BSA Free [NBP3-03924]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ZNRF3 (NP_001193927.1). MHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ZNRF3 Antibody - BSA Free
Western Blot: ZNRF3 AntibodyAzide and BSA Free [NBP3-03924]
Western Blot: ZNRF3 Antibody [NBP3-03924] - Analysis of extracts of Mouse testis, using ZNRF3 antibody at 1:3416 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.![Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]](https://resources.rndsystems.com/images/products/ZNRF3-Antibody-Immunohistochemistry-Paraffin-NBP3-03924-img0005.jpg "Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]")
Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]
Immunohistochemistry-Paraffin: ZNRF3 Antibody [NBP3-03924] - Rat lung using ZNRF3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]](https://resources.rndsystems.com/images/products/ZNRF3-Antibody-Immunohistochemistry-Paraffin-NBP3-03924-img0003.jpg "Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]")
Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]
Immunohistochemistry-Paraffin: ZNRF3 Antibody [NBP3-03924] - Human liver using ZNRF3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]](https://resources.rndsystems.com/images/products/ZNRF3-Antibody-Immunohistochemistry-Paraffin-NBP3-03924-img0004.jpg "Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]")
Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]
Immunohistochemistry-Paraffin: ZNRF3 Antibody [NBP3-03924] - Mouse spleen using ZNRF3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for ZNRF3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ZNRF3
Long Name
Zinc and Ring Finger 3
Alternate Names
BK747E2.3, KIAA1133, RNF203
Gene Symbol
ZNRF3
Additional ZNRF3 Products
Product Documents for ZNRF3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZNRF3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ZNRF3 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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