beta-Catenin is a downstream component of canonical Wnt signaling pathways. In unstimulated cells, beta-catenin is phosphorylated by the kinase GSK-3, and subsequently targeted for proteasomal degradation. Wnt binding to its receptors Frizzled and LRP-5/6, suppresses beta-catenin phosphorylation. beta-Catenin then accumulates and binds to LEF/TCF transcription factors resulting in the activation of Wnt target genes. The canonical Wnt pathway plays a role in cellular proliferation, differentiation, and motility, and has been implicated in tumor formation. Therefore, it is important to have quality research reagents for the study of beta-catenin and its activities. For an updated listing of other Wnt-related research reagents including antibodies, proteins, and ELISAs designed to study ligands, receptors, inhibitors, and intracellular signaling mediators, go to www.RnDSystems.com/go/Wnt
Figure 1. Lysates prepared from human MCF-7, HeLa, A431, SK-MEL-28, and DLD-2C2 cells were quantified with R&D Systems Human Total b-Catenin DuoSet® IC ELISA (Catalog # DYC1329). The same cell lysates were also immunoblotted (inset) with R&D Systems anti-b-catenin monoclonal antibody (Catalog # MAB1329). The DuoSet IC ELISA results correlate well with the total amount of beta-catenin detected by Western blot.
Figure 2. beta-Catenin was detected in sections of paraffin embedded human kidney cancer tissues using R&D Systems anti-human/mouse/rat beta-catenin monoclonal antibody (Catalog # MAB1329). Tissues were stained using R&D Systems anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue). The inset shows control staining without primary antibody.