Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, COMET

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 196621
View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222

Specificity

Detects rh beta -Catenin and endogenous human, mouse and rat beta -Catenin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat beta ‑Catenin Antibody

Detection of beta-Catenin in Human Colon Cancer via Multiplex Immunofluorescence staining on COMET™​

beta-Catenin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human beta-Catenin Monoclonal Antibody (Catalog # MAB1329) at 15 µg/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cell membrane. Protocol available in COMET™ Panel Builder.​
Detection of Human/Mouse/Rat beta -Catenin antibody by Western Blot.

Detection of Human/Mouse/Rat beta -Catenin by Western Blot.

Western blot shows lysates of Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
beta -Catenin antibody in Human Pancreas by Immunohistochemistry (IHC-P).

beta ‑Catenin in Human Pancreas.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat beta ‑Catenin Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Multiplex Immunofluorescence

15 µg/mL
Sample: Paraffin embedded tissue sections of Human Colon Cancer

Western Blot

2 µg/mL
Sample: Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB1329 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Catenin

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved in the canonical Wnt signal transduction pathway.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat)

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for Human/Mouse/Rat beta ‑Catenin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat beta ‑Catenin Antibody

For research use only

Citations for Human/Mouse/Rat beta ‑Catenin Antibody

Customer Reviews for Human/Mouse/Rat beta ‑Catenin Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Human/Mouse/Rat beta-Catenin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse/Rat beta ‑Catenin Antibody MAB1329
  • Human/Mouse/Rat beta -Catenin Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018

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Protocols

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