Human Total beta-Catenin DuoSet IC ELISA
Human Total beta-Catenin DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Complete Your Research
Complete Your Experiment
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Kit Content
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: beta-Catenin
beta-Catenin, also known as CTNNB1, is a cadherin-associated protein that is involved in the regulation of cell adhesion. This multifunctional protein is also implicated in the canonical Wnt signal transduction pathway, acting as a transcriptional co-activator. Nuclear beta-Catenin activates the TCF/LEF family of transcription factors, inducing the transcription of pro-mitotic genes.
Citations for Human Total beta-Catenin DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
Protein arginine methyltransferase 5 (PRMT5) activates WNT/&beta-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3
Authors: H Shailesh, KS Siveen, S Sif
Journal of Cellular and Molecular Medicine, 2021;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Rapid Osteogenic Enhancement of Stem Cells in Human Bone Marrow Using a Glycogen-Synthease-Kinase-3-Beta Inhibitor Improves Osteogenic Efficacy In Vitro and In Vivo
Authors: BH Clough, S Zeitouni, U Krause, CD Chaput, LM Cross, AK Gaharwar, CA Gregory
Stem Cells Transl Med, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Capture -
Comparison of Anticancer Effects of Carbamazepine and Valproic Acid
Authors: L Akbarzadeh, T Moini Zanj, M Sabetkasae
Iran Red Crescent Med J, 2016;18(10):e37230.
Species: Human
Sample Types: Cell Lysates
-
Incomplete epithelial-mesenchymal transition in p16-positive squamous cell carcinoma cells correlates with beta-catenin expression.
Authors: Umbreit C, Flanjak J, Weiss C, Erben P, Aderhold C, Faber A, Stern-Straeter J, Hoermann K, Schultz J
Anticancer Res, 2014;34(12):7061-9.
Species: Human
Sample Types: Cell Culture Supernates
-
Pharmaceutical inhibition of glycogen synthetase kinase-3beta reduces multiple myeloma-induced bone disease in a novel murine plasmacytoma xenograft model.
Authors: Gunn WG, Krause U, Lee N, Gregory CA
Blood, 2011;117(5):1641-51.
Species: Mouse
Sample Types: Tissue Homogenates
-
The Metastasis-Associated microRNA miR-516a-3p Is a Novel Therapeutic Target for Inhibiting Peritoneal Dissemination of Human Scirrhous Gastric Cancer.
Authors: Takei Y, Takigahira M, Mihara K, Tarumi Y, Yanagihara K
Cancer Res., 2011;71(4):1442-1453.
Species: Human
Sample Types: Cell Lysates
-
Carrageenan reduces bone morphogenetic protein-4 (BMP4) and activates the Wnt/beta-catenin pathway in normal human colonocytes.
Authors: Bhattacharyya S, Borthakur A, Dudeja PK, Tobacman JK
Dig. Dis. Sci., 2007;52(10):2766-74.
Species: Human
Sample Types: Cell Lysates
-
Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway.
Authors: Matushansky I, Hernando E, Socci ND, Mills JE, Matos TA, Edgar MA, Singer S, Maki RG, Cordon-Cardo C
J. Clin. Invest., 2007;117(11):3248-57.
Species: Human
Sample Types: Cell Lysates
-
Quantitative in situ analysis of beta-catenin expression in breast cancer shows decreased expression is associated with poor outcome.
Authors: Dolled-Filhart M, McCabe A, Giltnane J, Cregger M, Camp RL, Rimm DL
Cancer Res., 2006;66(10):5487-94.
Species: Human
Sample Types: Cell Lysates
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human Total beta-Catenin DuoSet IC ELISA
There are currently no reviews for this product. Be the first to review Human Total beta-Catenin DuoSet IC ELISA and earn rewards!
Have you used Human Total beta-Catenin DuoSet IC ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
