APP Antibody (6E10) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62566

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunoprecipitation, Electron Microscopy

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # 6E10

Format

Azide and BSA Free
View all APP Primary Antibodies »

Product Specifications

Immunogen

Amyloid-beta peptide

Specificity

This antibody binds to amino acid residues 1-16 of beta amyloid.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for APP Antibody (6E10) - Chimeric - Azide and BSA Free

Western Blot: APP Antibody (6E10)ChimericAzide and BSA Free [NBP2-62566]

Western Blot: APP Antibody (6E10)ChimericAzide and BSA Free [NBP2-62566]

Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.
Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric - Azide and BSA Free [NBP2-62566]

Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric - Azide and BSA Free [NBP2-62566]

Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.
APP Antibody (6E10) - Chimeric - Azide and BSA Free

Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] -

Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.
APP Antibody (6E10) - Chimeric - Azide and BSA Free

Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] -

Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.

Applications for APP Antibody (6E10) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Electron Microscopy

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. APP Antibody (6E10) validated for Immunohistochemistry-Frozen from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-62566 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: APP

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A 4) protein has been found to be the principal constituent of senile plaques of AD's patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A 4 protein. Two variants of A 4 can be detected; A 1-40 "short-tailed" and A 1-42 "long-tailed" (3-4).

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

Additional APP Products

Product Documents for APP Antibody (6E10) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for APP Antibody (6E10) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for APP Antibody (6E10) - Chimeric - Azide and BSA Free

Customer Reviews for APP Antibody (6E10) - Chimeric - Azide and BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
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  • APP Antibody (6E10) - Chimeric - Azide and BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 06/20/2023
    Plaque staining in AD Mouse model (Hippocampus)-without formic acid pretreatment
    APP Antibody (6E10) - Chimeric - Azide and BSA Free NBP2-62566
  • APP Antibody (6E10) - Chimeric
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 04/24/2023
    6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model.
    Free-floating IHC. Staining at a dilution of 1:500,000. Antibody worked phenomenally!
    APP Antibody (6E10) - Chimeric - Azide and BSA Free NBP2-62566
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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