ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80582

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Bovine, Ferret, Primate, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # H1beta234

Format

Azide and BSA Free
View all ARNT/HIF-1 beta Primary Antibodies »

Product Specifications

Immunogen

ARNT/HIF-1 beta Antibody (H1beta234) was developed against a fusion protein containing amino acids 496-789 of human HIF-1 beta. [Uniprot: P27540]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

86.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free (NB100-124) is a monoclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234)Azide and BSA Free [NBP2-80582]

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234)Azide and BSA Free [NBP2-80582]

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and ARNT/HIF-1 beta antibody (H1beta234) [NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight for ARNT/HIF-1 beta antibody (H1beta234) is 86.6 kDa. Observed molecular weight ~ 90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582]

Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582]

Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with ARNT/HIF-1 beta antibody (H1beta234) at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582]

Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582]

Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582] - Staining of human glioblastoma multi-forme utilizing ARNT/HIF-1 beta antibody (H1beta234). Image from the standard format of this antibody.
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234)Azide and BSA Free [NBP2-80582]

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234)Azide and BSA Free [NBP2-80582]

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582] - Analysis of HIF-1 beta using ARNT/HIF-1 beta antibody (H1beta234) in HeLa nuclear extract. Image from the standard format of this antibody. Theoretical molecular weight 86.6 kDa. Observed molecular weight ~85 kDa.
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234)Azide and BSA Free [NBP2-80582]

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234)Azide and BSA Free [NBP2-80582]

Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582] - Western Blot analysis using ARNT/HIF-1 beta antibody (H1beta234), theoretical molecular weight 86.6 kDa. Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1A or HIF-2A expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1A-shRNA or HIF-2A-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. HIF-1B expression was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582]

Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582]

Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free [NBP2-80582] - Co-localization of HIF-2alpha with HIF-1beta. ICC/IF detecting indicated proteins using Abs labelled with AF 555 (red, pseudocolour) and AF 488 (green, pseudocolour). 'Merge': the red image superimposed onto the green image of the co-stained nuclear proteins. 'Coloc': the co-localization channel calculated using ImageJ plugin. White indicates pixels where both red and green signal is found (i.e. co-localization). 'Overlay': the co-localization image superimposed onto the merged image. Inlay is the magnified region (white square). White arrows highlight regions of co-localization. Scale bar, 5um. Abbreviations: RNAPII, RNA Polymerase II phospho-serine 5. Image collected and cropped by CiteAb from the following publication (https://rsob.royalsocietypublishing.org/lookup/doi/10.1098/rsob.160195), licensed under a CC-BY license. Image from the standard format of this antibody.

Applications for ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Gel Super Shift Assays

reported in scientific literature (PMID 11325839)

Immunoblotting

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 25343232)

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500 - 1:2000
Application Notes
In Western blot, a band at approximately 92 kDa is seen.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARNT/HIF-1 beta

Aryl hydrocarbon nuclear translocator (ARNT), also commonly known as Hypoxia-inducible factor 1-beta (HIF-1 beta), is a ubiquitously expressed transcription factor that is part of the basic-helix-loop-helix (bHLH)-Per-ARNT-Sim (PAS) family (1, 2). Human arnt is located on chromosome 1q21 and encodes a protein 789 amino acids (aa) in length with a theoretical molecular weight of 87 kDa (1). Structurally, ARNT has a DNA binding bHLH domain, two PAS domains required for dimerization, and a transactivation domain/PAC region (1). ARNT belongs to the Class II bHLH-PAS proteins and is able to homodimerize or heterodimerize with the Class I proteins including AHA, AHRR, HIF-1 alpha, HIF-2 alpha, NPAS1, and SIM1 (2). Dimerization allows for efficient DNA binding and regulation of their target genes (2).

ARNT has an important role in two specific signaling pathways - the aryl hydrocarbon receptor (AhR) and the hypoxia inducible factor (HIF) pathway (1). In the AhR pathway, AhR in the cytosol is typically inactive and bound to heat shock protein 90 (hsp90) (3). Upon activation and ligand binding by environmental pollutants such as dioxins, AhR is translocated to the nucleus, dissociates from hsp90, and dimerizes with ARNT, leading to binding to response elements and expression of target genes including monooxygenases (1, 3). In the HIF pathway, under hypoxia (low oxygen) conditions prolylhydroxylase domain (PHD) enzymes and factor inhibiting HIF (FIH) are inhibited. HIF-1 alpha (or HIF-2 alpha) accumulates and is transported to the nucleus where it heterodimerizes with ARNT, allowing for binding to target gene's hypoxia response element (HRE), recruitment of coactivators, and transcription (1, 3). HIF-induced gene transcription plays a large role in tumor progression by promoting invasion, metastasis, de-differentiation and altered metabolism, and angiogenesis (1). While HIF-1 alpha's stability is dependent upon oxygen conditions, HIF-1 beta is stable in both normoxia and hypoxia (1-3).

The bHLH-PAS family and ARNT have been linked with a variety of pathologies and diseases including cancer, metabolic diseases, autoimmune diseases, and psychiatric disorders (2). ARNT/AHR is expressed in the skin and its pathway activation enhances skin barrier function and epidermal terminal differentiation, thus AHR agonists are currently being used as therapeutics for atopic dermatitis and psoriasis (4). Accordingly, studies of Arnt-deficient mice show profound abnormalities in skin barrier function and keratinization (4). Additionally, studies suggest that ARNT plays an important role in diabetes and beta-cell function (5). Islets from patients with type 2 diabetes have a significantly decreased ARNT expression compared to glucose-tolerant control donors (5). Modulation and stimulation of the HIF pathway may be a potential therapeutic strategy for treating type 2 diabetes and metabolic syndrome (5).

Alternate names for ARNT/HIF-1 beta include aryl hydrocarbon receptor nuclear translocator, BHLHE2, class E basic helix-loop-helix protein 2, Dixon receptor nuclear translocator, Hypoxia-inducible factor 1-beta, nuclear translocator, and TANGO.

References

1. Mandl, M., & Depping, R. (2014). Hypoxia-inducible aryl hydrocarbon receptor nuclear translocator (ARNT) (HIF-1beta): is it a rare exception?. Molecular medicine (Cambridge, Mass.). https://doi.org/10.2119/molmed.2014.00032

2. Wu, D., & Rastinejad, F. (2017). Structural characterization of mammalian bHLH-PAS transcription factors. Current opinion in structural biology. https://doi.org/10.1016/j.sbi.2016.09.011

3. Esser, C., & Rannug, A. (2015). The aryl hydrocarbon receptor in barrier organ physiology, immunology, and toxicology. Pharmacological reviews.https://doi.org/10.1124/pr.114.009001

4. Furue, M., Hashimoto-Hachiya, A., & Tsuji, G. (2019). Aryl Hydrocarbon Receptor in Atopic Dermatitis and Psoriasis. International journal of molecular sciences. https://doi.org/10.3390/ijms20215424

5. Girgis, C. M., Cheng, K., Scott, C. H., & Gunton, J. E. (2012). Novel links between HIFs, type 2 diabetes, and metabolic syndrome. Trends in endocrinology and metabolism: TEM, https://doi.org/10.1016/j.tem.2012.05.003

Long Name

Aryl Hydrocarbon Receptor Nuclear Translocator

Alternate Names

HIF-1 beta, HIF1 beta, TANGO

Gene Symbol

ARNT

Additional ARNT/HIF-1 beta Products

Product Documents for ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for ARNT/HIF-1 beta Antibody (H1beta234) - Azide and BSA Free

Showing  1 - 4 的 4 FAQs Showing All

    • Q: For use in Western Blot with HIF-1 beta antibodies, what molecular weight of the band should I expect to see?
      A: The theoretical molecular weight determined by our technical team for ARNT/HIF-1 beta antibodies is 86.6 kDa.

    • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
      A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.

    • A: NBP2-80582 is the Azide and BSA Free version of NB100-124. These products are the same except NBP2-80582 contains no preservative and NB100-124 contains 0.05% Sodium Azide as a preservative.

    • A: Yes; here is a list of research areas that we have deemed appropriate for the target "ARNT/HIF-1 beta": Angiogenesis, Autophagy, Cancer, Cellular Markers, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators, Cardiovascular Biology, Lipid and Metabolism.
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