Caldesmon/CALD1 Antibody (h-CALD)

Novus Biologicals | Catalog # NBP2-44421

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # h-CALD
View all Caldesmon/CALD1 Primary Antibodies »

Product Specifications

Immunogen

Crude human uterus extract

Localization

Cytoplasmic

Marker

Smooth Muscle Marker

Specificity

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47816)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Caldesmon/CALD1 Antibody (h-CALD)

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (h-CALD).
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Analysis of Human uterus tissue using Caldesmon/CALD1 antibody (h-CALD). Image from verified customer review.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Human Uterus stained with Caldesmon Monoclonal Antibody (h-CALD).
Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Simple Western lane view shows a specific band for Caldesmon/CALD1 in 0.2 mg/ml of h. Aorta lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Simple Western lane view shows a specific band for Caldesmon/CALD1 in 0.2 mg/ml of h. Uterus lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Electropherogram image of the corresponding Simple Western lane. Caldesmon/CALD1 antibody was used at 10 ug/ml dilution of h. Aorta and h. Uterus lysates(s) respectively.

Applications for Caldesmon/CALD1 Antibody (h-CALD)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Aorta lysate(s), h. Uterus lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 140-150 kDa.

Reviewed Applications

Read 1 review rated 5 using NBP2-44421 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

UniProt

Q05682 (Human)

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody (h-CALD)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caldesmon/CALD1 Antibody (h-CALD)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Caldesmon/CALD1 Antibody (h-CALD) (1)

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  • Caldesmon/CALD1 Antibody (h-CALD)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Uterus tissue
    Species: Human
    Verified Customer | Posted 03/24/2022
    Uterus tissue
    Caldesmon/CALD1 Antibody (h-CALD) NBP2-44421

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