CASK Antibody

Novus Biologicals | Catalog # NBP2-76922

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CASK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human CASK aa 300-500. (SwissProt: O14936 Human; SwissProt: Q62915 Rat)

Localization

Plasma membrane. Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CASK Antibody

Western Blot: CASK Antibody [NBP2-76922]

Western Blot: CASK Antibody [NBP2-76922]

Western Blot: CASK Antibody [NBP2-76922] - Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.Positive control:Lane1: SiHaLane2: A549
Immunocytochemistry/ Immunofluorescence: CASK Antibody [NBP2-76922]

Immunocytochemistry/ Immunofluorescence: CASK Antibody [NBP2-76922]

Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-76922] - ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CASK antibody. Counter stained with hematoxylin.
Flow Cytometry: CASK Antibody [NBP2-76922]

Flow Cytometry: CASK Antibody [NBP2-76922]

Flow Cytometry: CASK Antibody [NBP2-76922] - Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: CASK Antibody [NBP2-76922]

Immunocytochemistry/ Immunofluorescence: CASK Antibody [NBP2-76922]

Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-76922] - ICC staining CASK in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CASK antibody. Counter stained with hematoxylin.
Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CASK antibody. Counter stained with hematoxylin.
Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922]

Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. Counter stained with hematoxylin.

Applications for CASK Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:500-1:1000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CASK

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Alternate Names

Calcium/calmodulin-dependent serine protein kinase, calcium/calmodulin-dependent serine protein kinase (MAGUK family), calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase, CAMGUK, CMG, EC 2.7.11, EC 2.7.11.1, FGS4CAGH39, FLJ22219, hCASK, LIN2FLJ31914, MICPCH, peripheral plasma membrane protein CASK, Protein lin-2 homolog, TNRC8, trinucleotide repeat containing 8

Gene Symbol

CASK

Additional CASK Products

Product Documents for CASK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CASK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CASK Antibody

There are currently no reviews for this product. Be the first to review CASK Antibody and earn rewards!

Have you used CASK Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies