CD24 Antibody (ML5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80636
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # ML5
Format
Azide and BSA Free
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Product Specifications
Immunogen
The immunogen was the full length version of the protein: CD24 (ML5)
Reactivity Notes
Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Novus Biologicals Mouse CD24 Antibody (ML5) - Azide and BSA Free (NB100-77903) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD24 Antibody (ML5) - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]](https://beta-resources.rndsystems.com/images/products/CD24-Antibody-ML5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80636-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]")
Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]
Immunocytochemistry/Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - MCF-7 breast cancer cells. Image from the standard format of this antibody.![Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]](https://beta-resources.rndsystems.com/images/products/CD24-Antibody-ML5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80636-img0002.jpg "Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]")
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - Analysis using the FITC conjugate of NB100-77903. Staining of Human peripheral blood lymphocytes stained ML5 FITC.![Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]](https://beta-resources.rndsystems.com/images/products/CD24-Antibody-ML5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80636-img0003.jpg "Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]")
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636]
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - Whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC Image from the standard format of this antibody.Applications for CD24 Antibody (ML5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
0.125 ug/10^6 cells
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
5-25 ug/ml
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD24
Alternate Names
CD24, CD24A
Gene Symbol
CD24
Additional CD24 Products
Product Documents for CD24 Antibody (ML5) - Azide and BSA Free
Product Specific Notices for CD24 Antibody (ML5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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