Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1088014
View all CD57 Primary Antibodies »

Product Specifications

Immunogen

Whole Cell
Accession # Q9P2W7

Specificity

Detects the CD57 carbohydrate epitope in Human PBMCs

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for CD57 Antibody (1088014) [DyLight 350]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD57

The CD57 antigen, also referred to as human natural killer-1 (HNK-1) and LEU-7, is a carbohydrate epitope containing sulfated glucuronic acid that is expressed by many cell types within the nervous system (1,2). Cellular expression of CD57 changes during progression of neural development (1). The CD57 epitope is synthesized by enzymes including a sulfotransferase and one of two glucotransferases (GlcAT-P or GlcAT-S) (1,2). The structure of the CD57/HNK-1 epitope consists of 3-O-sulfated glucuronic acid (GlcA) attached to a terminal galactose (HSO3-3GlcAbeta1-3Galbeta1-4GlcNAc-R) (1,2). The CD57 epitope was first identified and a marker for HNK cells and its expression is associated with NK cell maturation (1-3).

CD57 expression has become a common pathological marker. CD57+ CD8+ T cell expression is observed in various cancers and is often linked to reduced survival (3). On the other hand, CD57+ NK cell expression is associated with less severe disease and better prognosis in cancer patients (3). CD57 is also associated with immune dysfunction and autoimmune disease (3,4). For example, T cell immunosenescence is characterized by an upregulation of CD57 along with KLRG-1 and a loss of CD27 and CD28 expression (4). Expanded autoreactive T cells are also related to diseases including multiple sclerosis (MS) and rheumatoid arthritis (RA) (3). Conversely, reduced numbers of CD57+ NK cells and NK cell cytotoxicity is observed in various autoimmune diseases such as apoptotic dermatitis and sjogren's syndrome (3).

References

1. Focosi D, Bestagno M, Burrone O, Petrini M. CD57+ T lymphocytes and functional immune deficiency. J Leukoc Biol. 2010;87(1):107-116. https://doi.org/10.1189/jlb.0809566

2. Morise J, Takematsu H, Oka S. The role of human natural killer-1 (HNK-1) carbohydrate in neuronal plasticity and disease. Biochim Biophys Acta Gen Subj. 2017;1861(10):2455-2461. https://doi.org/10.1016/j.bbagen.2017.06.025

3. Nielsen CM, White MJ, Goodier MR, Riley EM. Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease. Front Immunol. 2013;4:422. Published 2013 Dec 9. https://doi.org/10.3389/fimmu.2013.00422

4. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020;13(1):151. Published 2020 Nov 10. https://doi.org/10.1186/s13045-020-00986-z6

Long Name

Cluster of Differentiation 57

Alternate Names

Human Natural Killer-1, L2, LEU-7

Additional CD57 Products

Product Documents for CD57 Antibody (1088014) [DyLight 350]

Certificate of Analysis

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Product Specific Notices for CD57 Antibody (1088014) [DyLight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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