CD74 Antibody (PIN.1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80657

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PIN.1

Format

Azide and BSA Free
View all CD74 Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to residues 12-27 of CD74, human invariant chain (short form).

Epitope

The epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.

Reactivity Notes

Human. Does not react with mouse.

Localization

Type II membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CD74 Antibody (PIN.1) - Azide and BSA Free (NB100-1985) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD74 Antibody (PIN.1) - Azide and BSA Free

Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]

Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]

Western Blot: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF). Image from the standard format of this antibody.
Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Immunocytochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.
Flow Cytometry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Flow Cytometry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Flow Cytometry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining. Image from the standard format of this antibody.
Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]

Western Blot: CD74 Antibody (PIN.1)Azide and BSA Free [NBP2-80657]

Western Blot: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74. Image from the standard format of this antibody.
Immunohistochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Immunohistochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657]

Immunohistochemistry: CD74 Antibody (PIN.1) - Azide and BSA Free [NBP2-80657] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration. Image from the standard format of this antibody.

Applications for CD74 Antibody (PIN.1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1 ug/ml
Application Notes
In Simple Western only 10-15 ul of the recommended dilution is used per data point. In WB this detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD74

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Gene Symbol

CD74

Additional CD74 Products

Product Documents for CD74 Antibody (PIN.1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD74 Antibody (PIN.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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