CDK2 [p Thr14] Antibody (24GB4320)

Novus Biologicals | Catalog # NBP3-50790

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4320
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-CDK2 (Thr14) (Uniprot: P24941)

Modification

p Thr14

Specificity

This antibody detects endogenous levels of CDK2 only when phosphorylated at T14

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDK2 [p Thr14] Antibody (24GB4320)

CDK2 [p Thr14] Antibody (24GB4320)

Immunocytochemistry/ Immunofluorescence: CDK2 [p Thr14] Antibody (24GB4320)[NBP3-50790] -

Immunocytochemical staining of HAP-1 cells with CDK2 antibody (1:1,000). Nuclei were stained blue with DAPI;CDK2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
CDK2 [p Thr14] Antibody (24GB4320)

Flow Cytometry: CDK2 [p Thr14] Antibody (24GB4320)[NBP3-50790] -

Flow cytometric analysis of CDK2expression in HAP-1 cells using CDK2 antibody (1:2,000). Green, isotype control; red, Phospho-CDK2 (Thr14).
CDK2 [p Thr14] Antibody (24GB4320)

Western Blot: CDK2 [p Thr14] Antibody (24GB4320)[NBP3-50790] -

Western blotting analysis using CDK2 (Thr14) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for CDK2 [p Thr14] Antibody (24GB4320)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 [p Thr14] Antibody (24GB4320)

Certificate of Analysis

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Product Specific Notices for CDK2 [p Thr14] Antibody (24GB4320)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Akt Signaling Pathway
Akt Signaling Pathway Thumbnail