CSNK1A1/CSNK1A1L Antibody (PSH04-58)
Novus Biologicals | Catalog # NBP3-33146
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH04-58 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within human CSNK1A1 aa 1-337 / 337. (Uniprot: P48729)
Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, centromere, kinetochore, Nucleus speckle, cilium basal body, spindle.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CSNK1A1/CSNK1A1L Antibody (PSH04-58)
Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]
Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - CSNK1A1/CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-33146 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33146 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: HeLa cell lysate (input)
Lane 2: NBP3-33146 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of NBP3-33146 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 11 seconds; ECL
Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]
Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33146) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]
Immunocytochemistry/ Immunofluorescence: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunocytochemistry analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Flow Cytometry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]
Flow Cytometry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Flow cytometric analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L.Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
![CSNK1A1/CSNK1A1L Antibody (PSH04-58) Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]](https://resources.rndsystems.com/images/products/nbp3-33146_-western-blot-639204597488228483.jpg "Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]")
Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]
Western blot analysis on different lysates with NBP3-33146 at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse brain tissue lysate (40 µg/Lane) Lane 10: Rat brain tissue lysate (40 µg/Lane) Lane 11: Mouse kidney tissue lysate (40 µg/Lane) Lane 12: Rat kidney tissue lysate (40 µg/Lane) Lane 13: Mouse spleen tissue lysate (40 µg/Lane) Lane 14: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 39 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33146 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Applications for CSNK1A1/CSNK1A1L Antibody (PSH04-58)
Application
Recommended Usage
Flow Cytometry
1:1000
Immunocytochemistry/ Immunofluorescence
1:500
Immunohistochemistry-Paraffin
1:1000
Immunoprecipitation
1-2ug/sample
Western Blot
1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CSNK1A1/CSNK1A1L
Alternate Names
casein kinase I isoform alpha, casein kinase I isoform alpha-like, CK1, CKI-alpha-like
Gene Symbol
CSNK1A1
Additional CSNK1A1/CSNK1A1L Products
Product Documents for CSNK1A1/CSNK1A1L Antibody (PSH04-58)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CSNK1A1/CSNK1A1L Antibody (PSH04-58)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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