Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the center of human CXCR4 isoform b. The immunogen is located within amino acids 170-220 of CXCR4. Amino Acid Squence: DRYICDRFYPNDLWV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%), Sheep (86%).

Specificity

Human CXCR4 has four isoforms, including isoform a (356aa, 40.2kD), isoform b (352aa, 39.7kD), isoform c (423aa, 47.6kD), and isoform d (385aa, 43.4kD). This antibody detects human isoform b only, but not other human isoforms. Mouse CXCR4 has two isoforms, including isoform 1 (359aa, 40.4kD) and isoform 2 (357aa, 40.2kD). Rat CXCR4 has only one isoform identified so far (349aa, 39.4kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CXCR4 Antibody - BSA Free (NBP1-76480) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-CXCR4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CXCR4 Antibody - BSA Free

Western Blot: CXCR4 AntibodyBSA Free [NBP1-76480]

Western Blot: CXCR4 AntibodyBSA Free [NBP1-76480]

Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.
CXCR4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-76480] -

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-76480] - Figure 5 Immunofluorescence Validation of CXCR4
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 4 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.
Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NBP1-76480]

Immunohistochemistry-Paraffin: CXCR4 Antibody - BSA Free [NBP1-76480]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-76480] - Staining of CXCR4 in mouse spleen tissue with CXCR4 antibody at 5 ug/ml.
Western Blot: CXCR4 AntibodyBSA Free [NBP1-76480]

Western Blot: CXCR4 AntibodyBSA Free [NBP1-76480]

Western Blot: CXCR4 Antibody [NBP1-76480] - HeLa total cell lysate.
Western Blot: CXCR4 AntibodyBSA Free [NBP1-76480]

Western Blot: CXCR4 AntibodyBSA Free [NBP1-76480]

Western Blot: CXCR4 Antibody [NBP1-76480] - Animal Species ReactivityLoading: Lysates/proteins at 20 ug per lane.Antibodies: NBP1-77067 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
Knockdown Validated: CXCR4 Antibody - BSA Free [NBP1-76480]

Western Blot: CXCR4 Antibody - BSA Free [NBP1-76480]

Western Blot: CXCR4 Antibody [NBP1-76480] - Validation with CXCR4 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76480 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.

Applications for CXCR4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

4 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCR4

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Gene Symbol

CXCR4

UniProt

NP_003458

Additional CXCR4 Products

Product Documents for CXCR4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CXCR4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CXCR4 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR4 Antibody - BSA Free

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    • Q: Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
      A: CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.
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