Cytochrome P450 17A1 Antibody (OTI3F11)

Novus Biologicals | Catalog # NBP2-01151

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F11
View all Cytochrome P450 17A1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYP17A1(NP_000093) peoduced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 17A1 Antibody (OTI3F11)

Western Blot: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Western Blot: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human breast tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Flow Cytometry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151]

Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Cytochrome P450 17A1 Antibody (OTI3F11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-01151 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 17A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq].

Long Name

Steroid 17-alpha-hydroxylase/17,20 lyase

Alternate Names

CYP17, CYP17A1, CYPXVII, Cytochrome P450-C17, Cytochrome P450c17, EC 1.14.14.19, EC 1.14.14.32, S17AH

Entrez Gene IDs

1586 (Human)

Gene Symbol

CYP17A1

Additional Cytochrome P450 17A1 Products

Product Documents for Cytochrome P450 17A1 Antibody (OTI3F11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytochrome P450 17A1 Antibody (OTI3F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytochrome P450 17A1 Antibody (OTI3F11)

Customer Reviews for Cytochrome P450 17A1 Antibody (OTI3F11) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 的 2 reviews Showing All
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  • Cytochrome P450 17A1 Antibody (3F11)
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Pubertal testis
    Species: Rhesus Macaque
    Verified Customer | Posted 11/24/2017
    Specific positive staining on Leydig cells. Dil 1/250
    Cytochrome P450 17A1 Antibody (OTI3F11) NBP2-01151
  • Cytochrome P450 17A1 Antibody (3F11)
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human testis
    Species: Human
    Verified Customer | Posted 10/09/2017
    Thirteen year old human testis. Specific CYP17A1 immunoexpression in Leydig cells.
    primary abtibody diluted 1/250
    Cytochrome P450 17A1 Antibody (OTI3F11) NBP2-01151

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