Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-47940

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # OV-TL12/30

Format

Azide and BSA Free
View all Cytokeratin 7 Primary Antibodies »

Product Specifications

Immunogen

OTN 11, ovarian carcinoma cell line

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Localization

Cytoplasmic

Marker

Glandular and Transitional Epithelial Marker

Specificity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44814).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Formalin-fixed, paraffin-embeddedhuman prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Paraffin-embedded human salivary gland tissue. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate.Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/mL dilution of HeLa lysates(s) respectively.
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30)Azide and BSA Free [NBP2-47940]

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/mL of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

5 - 10 ul/million cells in 0.1ml

Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

1:100-1:200

Simple Western

5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 5 ug/mL
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949).

Reviewed Applications

Read 1 review rated 5 using NBP2-47940 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free

Customer Reviews for Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free (1)

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  • Cytokeratin 7 Antibody (OV-TL12/30)
    Name: Anonymous
    Application: Immunofluorescence - Paraffin
    Sample Tested: Salivary gland tissue
    Species: Human
    Verified Customer | Posted 09/23/2018
    Human salivary gland, Cytokeratin 7
    pH9 antigen retrieval
    Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free NBP2-47940

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