DAPI Solution

Novus Biologicals | Catalog # NBP2-31156

Novus Biologicals
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Key Product Details

Species

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Product Summary for DAPI Solution

DAPI is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. Dead cells which take up DAPI will fluoresce brightly around 461nm. DAPI may be excited by the UV or violet lasers although the UV laser is much more efficient. DAPI's emission spectra is very similar to the Pacific Blue (TM) dye.

Supplied at a concentration of 1 mg/ml.

Product Specifications

Applications

Immunocytochemistry/ Immunofluorescence (0.1-1.0ug/ml)

Reactivity Notes

Use in Human reported in scientific literature (PMID:33296752)

Scientific Data Images for DAPI Solution

Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156]

Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156]

Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.
Immunohistochemistry: DAPI Solution [NBP2-31156]

Immunohistochemistry: DAPI Solution [NBP2-31156]

Immunohistochemistry: DAPI Solution [NBP2-31156] - DAPI labelling in mouse brain. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156]

Immunocytochemistry/ Immunofluorescence: DAPI Solution [NBP2-31156]

Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.

Formulation, Preparation, and Storage

Formulation

Water

Preservative

No Preservative

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: DAPI Solution

Alternate Names

2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, 4,6-Diamidino-2-phenylindole dihydrochloride, DAPI dihydrochloride

Additional DAPI Solution Products

Product Documents for DAPI Solution

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DAPI Solution

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Customer Reviews for DAPI Solution (1)

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Customer Images

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  • Name: Arun Satelli
    Application: Immunocytochemistry
    Sample Tested: Human Cancer cell line LM7
    Species: Human
    Verified Customer | Posted 10/09/2014
    Dylight 550 and DAPI
    DAPI Solution NBP2-31156

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DAPI Solution

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    • A: The concentration of this solution is 1 mg/ml. 
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