DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-24275

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-YBX3-2D12

Format

Azide and BSA Free
View all DBPA Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human DBPA protein

Localization

Nucleus. Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Prepared in 10mM PBS WITHOUT BSA & azide.

Antibody without azide - store at -20 to -80C

Scientific Data Images for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free

Protein Array: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -

Analysis of Protein Array containing more than 19,000 full-length human proteins using DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free

Immunohistochemistry-Paraffin: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Flow Cytometry: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -

Flow cytometric analysis of PFA-fixed HeLa cells. DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/Immunofluorescence: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Applications for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml

Immunoprecipitation

1-2ug/100-500ug protein

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: DBPA

DBPA binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression

Alternate Names

cold shock domain protein A, Cold shock domain-containing protein A, cold-shock domain protein A, CSDA1, dbpA, DBPAcold-shock domain containing A1, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB, ZO-1-associated nucleic acid-binding protein, ZONAB

Gene Symbol

YBX3

Additional DBPA Products

Product Documents for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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