Dendritic Cell Marker Antibody (33D1) - BSA Free
Novus Biologicals | Catalog # NBP1-43284
![Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]](https://resources.rndsystems.com/images/products/Dendritic-Cell-Marker-Antibody-33D1-Flow-Cytometry-NBP1-43284-img0005.jpg "Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]")
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Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # 33D1
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this antibody was Dendritic Cell Marker.
Marker
Dendritic Cell Marker
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Description
The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Scientific Data Images for Dendritic Cell Marker Antibody (33D1) - BSA Free
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Analysis using the PE conjugate of NBP1-43284. Staining of BALB/c splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.125 ug of Rat IgG2b kappa Isotype Control PE (left) or 0.125 ug of Anti-Mouse Dendritic Cell Marker (33D1) PE (right).![Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]](https://resources.rndsystems.com/images/products/Dendritic-Cell-Marker-Antibody-33D1-Flow-Cytometry-NBP1-43284-img0001.jpg "Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]")
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse Dendritic Cell Marker (33D1) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable c![Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]](https://resources.rndsystems.com/images/products/Dendritic-Cell-Marker-Antibody-33D1-Flow-Cytometry-NBP1-43284-img0004.jpg "Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]")
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) - BSA Free [NBP1-43284]
Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Analysis using the Biotin conjugate of NBP1-43284. Staining of C57Bl/6 splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.25 ug of Rat IgG2b Isotype Control biotin (left) or 0.25 ug of bnti-Mouse Dendritic Cell Marker (33D1) Biotin (right) followed by Streptavidin PE.Applications for Dendritic Cell Marker Antibody (33D1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Application Notes
The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Dendritic Cell Marker
Alternate Names
DCs, Dendritic cell
Additional Dendritic Cell Marker Products
Product Documents for Dendritic Cell Marker Antibody (33D1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Dendritic Cell Marker Antibody (33D1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Dendritic Cell Marker Antibody (33D1) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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