dsDNA Antibody (DSD/4054R)

Novus Biologicals | Catalog # NBP3-07302

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # DSD/4054R
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Product Specifications

Immunogen

Nuclei of Burkitt's cells

Localization

Nuclear

Marker

Nuclear Marker

Specificity

This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This monoclonal antibody produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA % 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.(NBP3-08490)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for dsDNA Antibody (DSD/4054R)

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).
Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Formalin-fixed, paraffin-embedded human tonsil stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).
Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Lane view of AAV samples probed with NBP3-07302 in Probe 1 of RePlex with chemiluminescence detection and the anti-VP1/2/3 antibody in Probe 2 of RePlex with NIR detection
Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302]

Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Lane view of AAV9 samples probed with the anti-DNA antibody in Probe 1 of RePlex with chemiluminescence detection and the anti-VP1/2/3 antibody in Probe 2 of RePlex with NIR detection (left). Average peak areas of DNA detection were plotted from each serotype with error bars representing the standard deviations from the means (n=9) (right).

Applications for dsDNA Antibody (DSD/4054R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation: Tested in AAV purified from HEK293, separated by Size, Charge, antibody dilution of 2 ug/mL

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: dsDNA

Alternate Names

Double stranded DNA

Additional dsDNA Products

Product Documents for dsDNA Antibody (DSD/4054R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dsDNA Antibody (DSD/4054R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for dsDNA Antibody (DSD/4054R)

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Protocols

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