EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05771

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all EAAT1/GLAST-1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal portion of the human SLC1A3 protein (between amino acids 100-300) [UniProt P43003]

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Immunocytochemistry/ Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of SLC1A3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong cytoplasmic and membrane reactivity were observed.
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on Hek293 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NBP3-05771]

Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Applications for EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1 - 2 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EAAT1/GLAST-1

Human excitatory amino acid transporters (EAATs) are members of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is believed that these proteins reduce extracellular glutamate concentration, thereby modulating synaptic signaling. In addition, EAATs may also be important for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressed in the frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. EAAT1 shares some pharmacological similarities with EAAT3 and both are potent antagonists that appear to specifically block transport mediated by EAAT2. EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport by co-transporting sodium. EAAT1 is essential for terminating the postsynaptic action of glutamate, by rapidly removing released glutamate from the synaptic cleft.

Long Name

Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1

Alternate Names

EA6, GLAST-1, GLAST1, SLC1A3, GLAST

Gene Symbol

SLC1A3

Additional EAAT1/GLAST-1 Products

Product Documents for EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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