Endothelial Cells Antibody (RECA-1)

Novus Biologicals | Catalog # NB600-1388

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Goat, Rabbit, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RECA-1
View all Endothelial Cells Primary Antibodies »

Product Specifications

Immunogen

Mouse Endothelial Cells

Specificity

RECA-1 reacts with Rat Endothelial Cell Antigen (RECA), a cell surface antigen (MCA970R) on rat endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Endothelial Cells Antibody (RECA-1)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Endothelial Cells

RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

Alternate Names

Endothelium

Additional Endothelial Cells Products

Product Documents for Endothelial Cells Antibody (RECA-1)

Certificate of Analysis

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Product Specific Notices for Endothelial Cells Antibody (RECA-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endothelial Cells Antibody (RECA-1)

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Protocols

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FAQs

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