Fc epsilon RI Antibody (43160) - BSA Free
Novus Biologicals | Catalog # NBP1-43279
![Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]](https://resources.rndsystems.com/images/products/Fc-epsilon-RI-Antibody-43160-Flow-Cytometry-NBP1-43279-img0015.jpg "Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]")
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # 43160
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this antibody was FceRI alpha.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Scientific Data Images for Fc epsilon RI Antibody (43160) - BSA Free
Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]
Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Analysis using the FITC conjugate of NBP1-43279. Staining of the MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (filled histogram). Total![Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]](https://resources.rndsystems.com/images/products/Fc-epsilon-RI-Antibody-43160-Flow-Cytometry-NBP1-43279-img0001.jpg "Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]")
Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]
Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Surface staining of MC/9 cell line with Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.![Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]](https://resources.rndsystems.com/images/products/Fc-epsilon-RI-Antibody-43160-Flow-Cytometry-NBP1-43279-img0014.jpg "Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]")
Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]
Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Analysis using the Biotin conjugate of NBP1-43279. Staining of MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) Biotin (filled histogram)followed by Streptavidin PE. Total viable cells were useApplications for Fc epsilon RI Antibody (43160) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Fc epsilon RI
Alternate Names
alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide
Gene Symbol
FCER1A
Additional Fc epsilon RI Products
Product Documents for Fc epsilon RI Antibody (43160) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Fc epsilon RI Antibody (43160) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fc epsilon RI Antibody (43160) - BSA Free
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Q: Does this antibody work also on human tissue?
A: NBP1-43279 has only been tested and validated for use in mouse tissues. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.
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